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Safety Data Sheet for Guanidinium thiocyanate 0613 Material Safety Data Sheet or SDS for Guanidinium thiocyanate 0613 from Merck for download or viewing in the browser Catalog Number 0613 Product Name Guanidinium thiocyanate Select LanguagePoly(A) RNA was isolated directly from 4 M guanidine thiocyanate‐lysed Caenorhabditis elegans worm extracts as well as from lysed human K562 and vincristine‐resistant K562/VCR leukemia cells surrogate for guanidine in toxicological testing conducted in the 1980s Results are presented for the two compounds as a group Results A Description and uses of guanidine and guanidine hydrochloride Guanidine is a strong organic base that is found in the urine as a normal product of protein metabolism

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Guanidine thiocyanate solution-Guanidine thiocyanate (GuSCN) solution is used as a less onerous way compared with a frozen method to transport and stock fecal samples at room temperature for DNA analysis;Concerning researchers mixing bleach with RNA extraction kits waste These kits contain guanidine salts (eg, guanidine thiocyanate and guanidine hydrochloride) that may produce hazardous gases when combined with bleach (sodium hypochlorite) and/or strong acids Please reference the Bleach Incompatibility Information in Appendix 1 See also the

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Guanidine thiocyanate solution, BioUltra, for molecular biology, ~6 M in H2O MilliporeSigma Synonym Guanidine isothiocyanate, Guanidinium isothiocyanate, Guanidinium rhodanide, Guanidinium thiocyanate CAS Number Linear Formula NH 2 C(=NH)NH 2 HSCN Molecular Weight Beilstein Registry Number MDL For fecal storage, guanidine thiocyanate (GuSCN) is frequently used as a simple and safe means of storing feces at room temperature because GuSCNGuanidine thiocyanate is a potent chaotropic agent;

Guanidine thiocyanate is used as a part of a lysis buffer solution which isolates and extracts the viral RNA from a sample This allows for relatively quick diagnosis of the virus in a subject It is so effective in fact that guanidine thiocyanate is becoming increasingly indemand andCultured cells or tissues are homogenized in a denaturing solution containing 4 M guanidine thiocyanate The homogenate is mixed sequentially with 2 M sodium acetate (pH 4), phenol, and finally chloroform/isoamyl alcohol or bromochloropropane The resulting mixture is centrifuged, yielding an upper aqueous phase containing total RNAGuanidine thiocyanate is a potent chaotropic agent;

Guanidine thiocyanate ≥99 %, for biochemistry Highly chaotropic agent that potently destroys the threedimensional structure of proteins, leading to denaturation Guanidinium (iso)thiocyanate contains potent anionic as well as cationic groups, that form strong hydrogen bondsGuanidine thiocyanate solution BioUltra, for molecular biology, ~4 M in H2O; UN proper shipping nameCorrosive liquid, nos (phenol guanidine thiocyanate solution) Transport hazard class(es)8 Packing group II Environmental hazards yes Special precautions for userNot Applicable _____ Revision date25Apr17 Page 7 / 9

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Synonyms Guanidinium rhodanide,Guanidinium isothiocyanate,Guanidine isothiocyanate,Guanidinium thiocyanate;Guanidine thiocyanate Revision Date 22May19 Thiocyanic acid, compound with guanidine (11) Not listed Poecillia reticulata LC50=1 mg/L 96h Not listed EC50=424 mg/L 48h Persistence and DegradabilityMay persist Bioaccumulation/ AccumulationNo information available Mobility Will likely be mobile in the environment due to its water solubilityThus, by interfering with the hydrogen bond network in aqueous solutions, it has a destabilising effect on macromolecules, especially proteins It is commonly used in cells and the lysis processes in virus particles to extract nucleic acids, as it denatures RNAse

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1 Product Identification Synonyms Thiocyanic acid, compounded with guanidine (11);One milligram of tissue (or 10 6 cells) is added to 05 ml of guanidinium thiocyanate solution II and sonicated for 5 sec To this sequentially add 50 µl of 2 M sodium acetate, pH 40, 05 ml of phenol, equilibrated with water, and 01 ml of chloroform/isoamyl alcohol (24 1, v/v) with vigorous mixing between additionsGuanidine thiocyanate solution facilitates sample collection for plant rhizosphere microbiome analysis Xiaoxiao Sun 1, Meiling Wang , Lin Guo1,2, Changlong Shu , Jie Zhang and Lili Geng1 1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China

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Guanidine thiocyanate CAS No： Molecular Weight： Introduction Guanidine thiocyanate is one of the cheapest and easiest to prepare of the guanidine saltsGuanidine Thiocyanate is an ultrapure, molecular biology grade reagent It is free of detectable nuclease and protease activity and is a strong protein denaturant, as both the guanidinium cation and the thiocyanateDissolve 1 g of Thiocyanate Guanidine (GuSCN) in 100 ml of 01 M Tris hydrochloride, pH 64 Dissolution of GuSCN was facilitated by heating in a 60 to 65°C water bath under continuous shaking Buffers L2 is stable for at least 3 weeks at room temperature in the darkFind SigmaAldrich509 MSDS, related peerreviewed papers, technical documents, similar products &

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Find SigmaAldrich MSDS, related peerreviewed papers, technical documents, similar products & Two recent reports indicated guanidine thiocyanate (GTC) solution promotes a stability in composition and diversity of human fecal microbial analysis at room temperature form 1 day to 1 week (Nishimoto et al, 16;Check out our bestinclass prices for Guanidine Thiocyanate 6M Solution at AG Scientific, Inc With more than years of experience in the life science industry, we can supply the chemicals you need to accelerate your scientific discoveries

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Guanidine Thiocyanate is an ultrapure, molecular biology grade reagent It is free of detectable nuclease and protease activity and is a strong protein denaturant, as both the guanidinium cation and the thiocyanate anion are chaotropic agents It is provided in one bottle containing 500 g It is recommended for isolation of RNA, especially for tissues such as pancreas with high levels of Determination of Thiocyanate by Colorimetric To a 100 cc Nessler tube add 50 cc water and 5 cc (more if necessary) of the cyanide solution to be tested, then add 2 cc HCl and 10 cc of 5 per cent solution of ferric chloride, FeCl3 Mix, and dilute to the 100cc mark with water If Prussian blue should form on the addition of FeCl3, the solutionLinear Formula NH2C(=NH)NH2 HSCN;

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Guanidinium thiocyanate (GTC) Because GTC is highly toxic, it is advisable to use the entire amount as purchased from the manufacturer, rather than weighing a required amount Thus, with 1 kg of GTC add 2115 ml of 05 mol/l EDTA (50 mmol/l), pH 80, 5287 ml of 1 mol/l citrate, pH 70, 3525 ml of 30% sarcosyl (05%) and make up to 2115 lGuanidine monothiocyanate Company Identification Fisher Scientific 1 Reagent Lane Fair Lawn, NJ For information, callLinear Formula NH2C(=NH)NH2 HSCN;

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Avantor ®, a Fortune 500 company, is a leading global provider of missioncritical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industriesOur portfolio is used in virtually every stage of the most important research, development and production activities in the industries we serveUltraPure™ Guanidine Isothiocyanate Solution consists of 4 M guanidine isothiocyanate, 50 mM TrisHCl (pH 75), 25 mM EDTA It may be used as part of a protocol, based on the guanidine isothiocyanate procedure first described by Chirgwin et al (1) and later modified by Han et al (2), for isolating intact mRNA from tissues or cultured cellsMSDS Name Guanidine thiocyanate Catalog Numbers AC, AC, , , BP2211, BP Synonyms Thiocyanic acid, compd with guanidine (11);

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Guanidine thiocyanate melamine ammonia guanidine thiocyanate Prior art date Legal status (The legal status is an assumption and is not a legal conclusion Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed) Expired Lifetime Application number US0875A Inventor Nat H MarshGuanidine thiocyanate solution BioUltra, for molecular biology, ~6 M in H2O;Abstract As an alternative to phenol extraction for the isolation of RNA, homogenization in guanidine hydrochloride solutions is an inexpensive and convenient method For those samples with very high RNase content or for irreplaceable samples with unknown RNase content, guanidinium thiocyanate becomes the deproteinizing agent of choice

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Guanidium thiocyanate CAS No MolecularGuanidine thiocyanate solution facilitates sample collection for plant rhizosphere microbiome analysis PeerJ 19 Feb 19;7e6440 doi /peerj6440 eCollection 19 Authors Xiaoxiao Sun6 M Guanidine Thiocyanate Buffer Section 1 Identification Product Identifier Catalog Number G4000 Recommended use of the chemical and restrictions on use For research use only Not intended for human or animal diagnostic or therapeutic uses Manufacture/Supplier Details Alpha Teknova, Inc 2290 Bert Dr Hollister, CA

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 Guanidine thiocyanate is a general protein denaturant3 and inhibits bacterial growth3–5 We collected faecal samples before and after colonoscopy, and divided each into two parts one was stored frozen and the other at RTHosomi et al, 17)1 Prepare 25 ml of guanidine isothiocyanate/2mercaptoethanol (GuSCN/ME) solution by adding 2 ml of 2mercaptoethanol to 23 ml of Guanidine Isothiocyanate Solution in a 50 ml sterile, disposable polypropylene centrifuge tube Mix by inversion and chill on ice for 10 min 2

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Thus, by interfering with the hydrogen bond network in aqueous solutions, it has a destabilising effect on macromolecules, especially proteinsGuanidine exists protonated, as guanidinium, in solution at physiological pH Guanidinium chloride (also known as guanidine hydrochloride) has chaotropic properties and is used to denature proteins Guanidinium chloride is known to denature proteins with a linear relationship between concentration and free energy of unfoldingChemical Name Guanidine Thiocyanate Synonyms Guanidine Isothiocyanate CAS# EC# Molecular Formula C 2 H 6 N 4 S or NH 2 C(NH)NH 2 ·HSCN Molecular Weight g/mol SECTION 4 First Aid Measures 41 Description of Necessary First Aid Measures

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Guanidine thiocyanate production guanidinium thiocyanate filtering reaction Prior art date Legal status (The legal status is an assumption and is not a legal conclusion Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed) Pending Application number CN OtherGuanidine Thiocyanate @ 2510% Ethanol @ 2510% NonHazardous Proprietary Ingredients @ Balance Chemical Hazardous WasteSynonyms Guanidine Isothiocyanate Formula NH 2 C(NH)NH 2 ·HSCN Molecular Weight g/mol CAS# EC# Component Classification Concentration Guanidine Thiocyanate Acute Tox 4;

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Synonyms Guanidinium rhodanide,Guanidinium isothiocyanate,Guanidine isothiocyanate,Guanidinium thiocyanate;The paper asks to prepare phenol mix of ''watersaturated phenol containing 35% (w/v) of guanidium thiocyanate'' I tried to dissolve 7g guanidium thiocyanate in (~ ml)Guanidinium Thiocyanate (GITC) Lysis buffers for nucleic acid extraction Guanidine thiocyanate is a potent chaotropic agent;

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GTI Laboratories Supplies Product identifiers Product name Product Number Brand CASNo 12 Relevant identified uses of the substance or mixture and uses advised against Identified uses Laboratory chemicals, Manufacture of substancesGL Guanidine Thiocyanate 4M Solution, 4 LitersThus, by interfering with the hydrogen bond network in aqueous solutions, it has a destabilizing effect on macromolecules, especially proteins It is commonly used in cells and the lysis processes in virus particles to extract nucleic acids, as it denatures RNAse and DNAse enzymes1 that would

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Guanidine thiocyanate, BioUltra, for molecular biology, >=990% (AT) Guanidine thiocyanate solution, BioUltra, for molecular biology, ~4 M in H2O Guanidine thiocyanate solution, BioUltra, for molecular biology, ~6 M in H2OH302 H312 H332, H314, H412 ≤100% Section 4 First Aid MeasuresHowever, optimal methods to measure fecal bacterial composition in GuSCN solution remain to

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